Publications 2012

Neu Ursula, Holger Hengel, Bärbel S. Blaum, Rachel M. Schowalter, Dennis Macejak, Akihiro Imamura, Makoto Kiso, Niklas Arnberg, Robert L. Garcea, Thomas Peters, Christopher B. Buck, and Thilo Stehle. Glycans as receptors for Merkel Cell Polyomavirus. Structures of Merkel Cell Polyomavirus VP1 complexes define a sialic acid binding site required for infection. PloS Pathogens. 2012;8(7):e1002738.

Arnberg Niklas. Adenovirus receptors: implications for targeting of viral vectors.Trends Pharmacol Sci. 2012 33:442-448.

Klinth, J. E.; Pinkner, J. S.; Hultgren, S. J.; Almqvist, F.; Uhlin, B-E.; Axner, O., Impairment of the biomechanical compliance of P pili - A novel means to inhibit uropathogenic bacterial infections?. Eur. Biophys. J., 2012, 41(3), 285-295.

Chorell, E.; Pinkner, J. S.; Bengtsson, C.; Sainte-Luce Banchelin, T.; Edvinsson, S.; Linusson, A.; Hultgren, S. J. *; Almqvist, F. *, Mapping pilicide anti-virulence effect in E. coli, a comprehensive structure-activity study. Bioorg. Med. Chem., 2012, 20 (9), 3128-3142.

Chorell, E.; Pinkner, J. S.; Bengtsson, C.; Cusumano, C. K.; Edvinsson, S.; Rosenbaum, E.; Johansson, B-Å. L.; Hultgren, S. J. *; Almqvist, F. *, Design and synthesis of fluorescently labeled pilicides and curlicides: bioactive tools to study bacterial virulence mechanisms. Chemistry-A European Journal, 2012, 18 (15), 4522-4532 (Highlighted with a frontispiece cover)

Horvath, I.; Weise, C. F.; Andersson, E. K.; Chorell, E.; Sellstedt,, M.; Bengtsson, C.; Olofsson, A.; Hultgren, S. J.; Chapman, M. R.; Wolf-Watz, M.; *; Almqvist, F. *; Wittung-Stafshede, P.*, Mechanisms of protein oligomerization: Inhibitor of functional amyloids templates -synuclein fibrillation. J. Am. Chem. Soc. 2012, 134 (7), 3439-3444.

Hammer, N. D.; McGuffie, B. A.; Zhou, Y.; Badtke, M. P.; Reinke, A. A.; Brännström, K.; Gestwicki, J. E.; Olofsson, A.; Almqvist, F.; Chapman, M. R., The C-terminal repeating units of CsgB direct bacterial functional amyloid nucleation. J. Mol. Biol. 2012, 422 (3), 376-389. (*)

Bengtsson, C.; Lindgren, A.; Uvell, H.; Almqvist, F.*, Design, synthesis and evaluation of triazole functionalized thiazolino/thiazolo ring-fused 2-pyridones as antibacterial agents. Eur. J. Med. Chem. 2012, 54, 637-646,

Sellstedt, M.; Prasad, G. K.; Krishnan, K.; Almqvist, F.*, Directed diversity-oriented synthesis. Ring-fused 5 to 10-membered rings from a common peptidomimetic 2-pyridone precursor. Tetrahedron Lett., 2012, 45, 6022-6024.

Zhou, Y.; Smith, D.; Leong, B. J.; Brännström, K.; Almqvist, F.; Chapman, M. R., Promiscuous cross-seeding between bacterial amyloids promotes interspecies biofilms. J. Biol. Chem., 2012, 287 (42), 35092-35103.

Poli, J., Tsaponina, O., Crabbé, L., Keszthelyi, A., Pantesco, V., Chabes, A., Lengronne, A., & Pasero, P. (Epub 2012 Jan 10) dNTP pools determine fork progression and origin usage under replication stress. EMBO J. 31, 883-94.

Davidson, M.B., Katou, Y., Keszthelyi, A., Sing, T.L., Xia, T., Ou, J., Vaisica, J.A., Thevakumaran, N., Marjavaara, L., Myers, C.L., Chabes, A., Shirahige, K., & Brown, G.W. (Epub 2012 Jan 10) Endogenous DNA replication stress results in expansion of dNTP pools and a mutator phenotype. EMBO J. 31, 895-907.

Sánchez, A., Sharma, S., Rozenzhak, S., Roguev, A., Krogan, N.J., Chabes, A., & Russell, P. (Epub 2012 Aug 27) Replication fork collapse and genome instability in a deoxycytidylate deaminase mutant. Mol Cell Biol. 32, 4445-4454.

Thanikkal E.J., Mangu J.C., Francis M.S. (2012) Interactions of the CpxA sensor kinase and cognate CpxR response regulator from Yersinia pseudotuberculosis. BMC Res Notes. 5: 536, doi: 10.1186/1756-0500-5-536

Liu J., Thanikkal E.J., Obi I.R., Francis M.S. (2012) CpxR~P mediates repression of Ysc-Yop type III secretion by Yersinia pseudotuberculosis. Res Microbiol. 163: 518-530.

Costa T.R., Amer A.A., Fällman M., Fahlgren A., Francis M.S. (2012) Coiled‐coils in the YopD translocator family: a structure unique to the YopD N‐terminus contributes to full virulence of pathogenic Yersinia sp. Infect Genet Evol. 12: 1729-1742.

R. Marcinowska, J. Trygg, H. Wolf-Watz, T. Mortiz and I. Surowiec, Optimization of a sample preparation method for the metabolomic analysis of clinically relevant bacteria, J Microbiol Methods 87 (2012), no. 1, 24-31.

S. Frost, O. Ho, F. H. Login, C. F. Weise, H. Wolf-Watz and M. Wolf-Watz, Autoproteolysis and intramolecular dissociation of yersinia yscu precedes secretion of its c-terminal polypeptide yscu(cc), PLoS One 7 (2012), no. 11, e49349.

T. R. Costa, A. A. Amer, S. I. Farag, H. Wolf-Watz, M. Fallman, A. Fahlgren, T. Edgren and M. S. Francis, Type iii secretion translocon assemblies that attenuate yersinia virulence, Cell Microbiol (2012).

K. Bohme, R. Steinmann, J. Kortmann, S. Seekircher, A. K. Heroven, E. Berger, F. Pisano, T. Thiermann, H. Wolf-Watz, F. Narberhaus and P. Dersch, Concerted actions of a thermo-labile regulator and a unique intergenic rna thermosensor control yersinia virulence, PLoS Pathog 8 (2012), no. 2, e1002518.

Netterling S, Vaitkevicius K, Nord S, Johansson J#. A Listeria monocytogenes RNA Helicase essential for growth and ribosomal maturation at low temperatures uses its C-terminus for appropriate interaction with the ribosome. J Bacteriol. 194:4377-4385. 2012.

Loh, E., Memarpor, F., Vaitkevicius, K., Kallipolitis, B., Johansson, J# and Sondén, B. An unstructured 5´-coding region of the prfA mRNA is required for efficient translation. Nucleic Acids Research, 40:1818-1827. 2012.

J. E. Klinth, J. S. Pinkner, S. J. Hultgren, F. Almqvist, B. E. Uhlin, and O. Axner, "Impairment of the biomechanical compliance of P pili: a novel means of inhibiting uropathogenic bacterial infections?" Euro. Biophys. J. with Biophys. Lett. 41, 285-295 (2012).

I. Silander, P. Ehlers, J. Wang, and O. Axner, "Frequency Modulation Background Signals from Fiber-based Electro Optic Modulators are Caused by Crosstalk," J. Opt. Soc. Am. B 29, 916-923 (2012).

P. Ehlers, I. Silander, J. Wang, and O. Axner, "Fiber-laser-based noise-immune cavity-enhanced optical heterodyne molecular spectrometry instrumentation for Doppler-broadened detection in the 10−12 cm−1 Hz−1∕2 region," J. Opt. Soc. Am. B 29, 160011 (2012).

J. E. Klinth, M. Castelain, B. E. Uhlin, and O. Axner, "The Influence of pH on the Specific Adhesion of P Piliated Escherichia coli," Plos One 7, e38548 (2012), doi:10.1371/journal.pone.0038548.

J. Westberg, J. Wang, and O. Axner, "Fast and non-approximative methodology for calculation of wavelength-modulated Voigt lineshape functions suitable for rreal-time curve fiting," J. Quant. Spectrosc. Radiat. Tr 113, 2049-2057

J. Y. Wang, P. Ehlers, I. Silander, and O. Axner, "Speed-dependent effects in dispersion mode of detection and in noise-immune cavity-enhanced optical heterodyne molecular spectrometry: experimental demonstration and validation of predicted line shape," J. Opt. Soc. Am. B 29, 2980-2989 (2012).

J. Y. Wang, P. Ehlers, I. Silander, J. Westberg, and O. Axner, "Speed-dependent Voigt dispersion line-shape function: applicable to techniques measuring dispersion signals," J. Opt. Soc. Am. B 29, 2971-2979 (2012).

Skogman BH., Hellberg S, Ekerfelt C, Jenmalm M, Forsberg P, Ludvigsson J, Bergström S and Ernerudh J. Adaptive and innate immune responsiveness to Borrelia burgdorferi sensu lato in exposed asymptomatic children and children with previous clinical Lyme Borreliosis. 2012. Clinical and Developmental Immunology, doi:10.1155/2012/294587

Elbir H, Gimenez G, Robert C, Bergström S, Cutler S, Raoult D and Drancourt M. 2012 Complete genome sequence of Borrelia crocidurae . Accepted for publication in the Genome Announcement section, J. Bacteriol 194:3723-4.

Thein M, Bonde M, Bunikis I, Denker K, Sickmann A Bergström S, and Benz R. DipA, a pore-forming protein in the outer membrane of Lyme disease spirochetes exhibits specificity for the permeation of dicarboxylates. 2012, PLoS One ;7(5):e36523. doi: 10.1371/journal.pone.0036523. Epub 2012 May 10.

Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA and Charpentier E. 2012. A programmable dual-RNA guided DNA endonuclease in adaptive bacterial immunity. Science (Article) 337(6096):816-821.

Edgren T, Forsberg Å, Rosqvist R, Wolf-Watz H (2012) Type III secretion in Yersinia: injectisome or not? PLoS Pathog 8(5): e1002669.

Bjarnsholt T, Høiby N, Donelli G, Imbert C, Forsberg Å. 2012. Understanding biofilms--are we there yet? FEMS Immunol Med Microbiol.;65(2):125-6

Balanova L, Mann BF, Cerveny L, Alley WR Jr, Chovancova E, Forslund AL, Salomonsson EN, Forsberg Å, Damborsky J, Novotny MV, Hernychova L, Stulik J. Characterization of protein glycosylation in Francisella tularensis subsp. holarctica: identification of a novel glycosylated lipoprotein required for virulence. Mol Cell Proteomics. 2012 11(7): M111.015016. doi: 10.1074/mcp.M111.015016. Epub 2012

Paracuellos, P., Öhman, A., Sauer-Eriksson, A.E. & Uhlin, B.-E. Expression and purification of SfaXII, a protein involved in regulating adhesion and motility genes in extraintestinal pathogenic Escherichia coli (2012) Protein expression and purification, 86(2):127-134

Eriksson, J., Grundström, C., Sauer-Eriksson, A.E., Sauer, U.H., Wolf-Watz, H. & Elofsson, M. Small molecule screening for inhibitors of the YopH phosphatase of Yersinia pseudotuberculosis. (2012) Adv. Exp. Med. Biol. 954:357-363

Hogg, M., Sauer-Eriksson, A.E. & Johansson, E. Promiscuous DNA synthesis by human DNA polymerase θ.(2012) Nucleic Acids Research, 40(6):2611-22

J. M. Hillgren, C. T. Öberg, M. Elofsson. Syntheses of pseudoceramines A−D and a new synthesis of spermatinamine, bromotyrosine natural products from marine sponges. Organic & Biomolecular Chemistry, 10 (2012) 1246-1254.

M. Gabrielsen, K. S. H. Beckham, V. A. Feher, C. E. Zetterström, D. Wang,
S. Müller, M. Elofsson, R. E. Amaro, O. Byron, A. J. Roe. Structural characterisation of thiol peroxidase from Yersinia pseudotuberculosis reveals insights into the binding of salicylidene acylhydrazide compounds. PLoS One, 7 (2012) e32217.

C. T. Öberg, M. Strand, E. K. Andersson, K. Edlund, N. Phuong, Y.-F. Mei, G. Wadell, Mikael Elofsson. Synthesis, biological evaluation, and structure-activity relationships of 2-[2-(benzoylamino) benzoylamino]-benzoic acid analogs as inhibitors of adenovirus replication. Journal of Medicinal Chemistry, 55 (2012) 3170-3181.

P. -A. Enquist, Å. Gylfe, U. Hägglund, P. Lindström, H. Norberg-Scherman, C. Sundin, M. Elofsson. Derivatives of 8-hydroxyquinoline – antibacterial agents that target intra- and extracellular pathogens. Bioorganic and Medicinal Chemistry Letters, 22 (2012) 3550-3553.

T. Ur-Rehman, A. Slepenkin, H. Chu, A. Blomgren, M. K. Dahlgren, C. E. Zetterström, E. M. Peterson, M. Elofsson, Å. Gylfe. Pre-clinical pharmacokinetics and anti-chlamydial activity of salicylidene acylhydrazide inhibitors of bacterial type III secretion. Journal of Antibiotics, 65 (2012) 397-404.

D. N. Forthal, T. B. Phan, A. V. Slepenkin, G. Landucci, H. Chu, M. Elofsson, E. M. Peterson. In vitro anti-HIV-1 activity of salicylidene acylhydrazide compounds that also inhibit sexually transmitted bacterial pathogens. International Journal of Antimicrobial Agents, 40 (2012) 354-360.

C. D. Andersson, T. Karlberg, T. Ekblad, A. E. G. Lindgren, A-.G Thorsell, S. Spjut, M. S. Niemiec, P. Wittung-Stafshede, J. Weigelt, M. Elofsson, Herwig Schüler,
A. Linusson. Discovery of Ligands for the human diphtheria toxin-like ADP-Ribosyltransferases ARTD7 and ARTD8 via Docking-Based Virtual Screening. Journal of Medicinal Chemistry, 55 (2012) 7706-7708.

M. Strand, K. Islam, K. Edlund, C. T. Öberg, A. Allard, T. Bergström, Y.-F. Mei, M. Elofsson, G. Wadell. 2-[4,5-Difluoro-2-(2-fluorobenzoylamino)-benzoylamino] benzoic acid, an antiviral compound with activity against acyclovir-resistant isolates of herpes simplex type -1 and -2. Antimicrobial Agents & Chemotherapy, 56 (2012) 5735-5743.

Nakao R, Ramstedt M, Wai SN, Uhlin BE. (2012) Enhanced Biofilm Formation by Escherichia coli LPS Mutants Defective in Hep Biosynthesis. PLoS ONE, 2012;7(12):e51241.

Valeru SP, Wai SN, Saeed A, Sandström G, Abd H. (2012) ToxR of Vibrio cholerae affects biofilm, rugosity and survival with Acanthamoeba castellanii. BMC Res Notes. 16;5:33

Hedberg ME, Moore ER, Svensson-Stadler L, Hörstedt P, Baranov V, Hernell O, Wai SN, Hammarström S, Hammarström ML. (2012) Lachnoanaerobaculum a new genus in Lachnospiraceae; characterization of Lachnoanaerobaculum umeaense gen. nov., sp. nov., isolated from human small intestine, Lachnoanaerobaculum orale gen. nov., sp. nov., isolated from saliva and reclassification of Eubacterium saburreum (Prevot) Holdeman and Moore 1970 as Lachnoanaerobaculum saburreum comb. nov. Int J Syst Evol Microbiol. Int J Syst Evol Microbiol. 62(Pt 11):2685-90.

Ishikawa T, Sabharwal D, Bröms J, Milton DL, Sjöstedt A, Uhlin BE, Wai SN. (2012) Pathoadaptive conditional regulation of the type VI secretion system in Vibrio cholerae O1 strains. Infect Immun. 80(2):575-84.

Rompikuntal PK, Thay B, Khan MK, Alanko J, Penttinen AM, Asikainen S, Wai SN, Oscarsson J. (2012) Perinuclear localization of internalized outer membrane vesicles carrying active cytolethal distending toxin from Aggregatibacter actinomycetemcomitans. Infect Immun. 80(1):31-42.

Westermark, L, A. Fahlgren, and M. Fällman. Massive immune response to diphtheria toxin mediated depletion complicates the use of the CD11c-DTR model during infection (2012) Microb Pathog. 53(3-4):154-161

Kristoffer Lindell, Anna Fahlgren, Erik Hjerde, Nils-Peder Willassen, Maria Fällman, and Debra L. Milton (2012) Lipopolysaccharide O-Antigen Prevents Phagocytosis of Vibrio anguillarum by Rainbow Trout (Oncorhynchus mykiss) Skin Epithelial Cells. PLoS ONE 7(5):e37678

Patrik Ryden, Susan Twine, Hua Shen, Gregory Harris, Wangxue Chen, Anders Sjöstedt, Wayne Conlan. (2012). Correlates of protection following vaccination of mice with mutants of Francisella tularensis subspecies tularensis strain, SCHU S4 that elicit varying degrees of immunity to systemic and respiratory challenge with wild-type bacteria. Molec. Immunol. 54:58-67.

Brooke A. Napier, Lena Meyer, James E. Bina, Mark A. Miller, Anders Sjöstedt, David S Weiss. (2012). A link between intraphagosomal biotin and rapid phagosomal escape in Francisella. Proc. Natl. Acad. Sci. 109:18084-18089.

Malin Vonkavaara, Shaikh Terkis Islam Pavel, Kathrin Hölzl, Roland Nordfelth, Anders Sjöstedt and Svenja Stöven. (2012). Francisella is sensitive to insect antimicrobial peptides. In the press. Innate Immunity.

S. M. Twine, E. Vinogradov, H. Lindgren, A. Sjöstedt, J. W. Conlan. (2012). Roles for wbtC, wbtI, and kdtA genes in lipopolysaccharide biosynthesis, protein glycosylation, virulence, and immunogenicity in Francisella tularensis strain SCHU S4. Pathogens. 1:12-29; doi:10.3390/pathogens1010012

Susan Twine, Hua Shen, Gregory Harris, Wangxue Chen, Anders Sjöstedt, Patrik Ryden, Wayne Conlan. (2012) BALB/c mice, but not C57BL/6 mice immunized with a ΔclpB mutant of Francisella tularensis subspecies tularensis are protected against respiratory challenge with wild-type bacteria; association of protection with post-vaccination and post-challenge immune responses. Vaccine. 30:3634-3645.

Jeanette E. Bröms, Lena Meyer, Moa Lavander, and Anders Sjöstedt. (2012). DotU and VgrG, core components of Type VI secretion systems, are essential for Francisella pathogenicity. PLoS One. 7:e34639.

Kjell Eneslätt, Monica Normark, Rafael Björk, Cecilia Rietz, Carl Zingmark, Lawrence A. Wolfraim, Svenja Stöven, and Anders Sjöstedt. (2012). Identification of polyfunctional human T cells specific for Francisella tularensis. PLoS One 7:e32367.

Marie Honn, Helena Lindgren, and Anders Sjöstedt. (2012). The role of MglA for adaptation to oxidative stress of Francisella tularensis LVS. BMC Microbiol. 12:14.

Patrik Rydén, Rafael Björk, Martina Schäfer, Jan Lundström, Bodil Petersén, Anders Lindblom, Mats Forsman, Anders Sjöstedt, and Anders Johansson. (2012). Swedish outbreaks of tularemia in man are explained by late summer mosquito prevalence. J. Inf. Dis. 205:297-304.

Achouiti, A., Vogl, T., Urban, C.F., Rohm, M., Hommes, T.J., van Zoelen, M.A., Florquin, S., Roth, J., van 't Veer, C., de Vos, A.F., van der Poll, T. (2012) Myeloid-related protein-14 contributes to protective immunity in gram-negative pneumonia derived sepsis. PLoS Pathog 8, e1002987.

Hosseinzadeh, A., Messer, P.K., Urban, C.F. (2012) Stable Redox-Cycling Nitroxide Tempol Inhibits NET Formation. Front Immunol 3, 391. Rohm, M., Lindemann, E., Hiller, E., Ermert, D., Lemuth, K., Trkulja, D., Sogukpinar, O., Brunner, H., Rupp, S., Urban, C.F., Sohn, K. (2012) A family of secreted pathogenesis-related proteins in Candida albicans. Mol Microbiol.

Andersson M, Björnhamn O, Svantesson M, Badahdah A, Uhlin BE, Bullitt E (2012) A structural basis for sustained bacterial adhesion – Biomechanical properties of CFA/I pili. J. Mol. Biol. 415:918-928.