Department of Molecular Biology and MIMS

Thesis Defence
Christopher Andersson

Regulatory pathways and virulence inhibition in Listeria monocytogenes

Supervisor: Jörgen Johansson

Faculty Examiner:
Professor Claude Parsot, Unite de Pathogenie Microbienne Moleculaire, Institute Pasteur, Paris

Place: KB3B1, Stora hörsalen, KBC-Building

MIMS- UCMR Seminar Medical Biochemistry and Biophysics Seminar

Speaker:
Oliver Billker
Wellcome Trust Sanger Institute, Cambridge, United Kingdom.

Title:
"Regulating a complex life cycle — New molecular tools reveal mechanisms of malaria parasite transmission by mosquitoes".

Host: Bernt Eric Uhlin

Place: Lilla hörsalen KB3A9, KBC

Department of Chemistry
Seminar
Speaker:
Professor Dr. Christian P. R. Hackenberger
Humboldt Universität zu Berlin
and
Leibniz-Institut für Molekulare Pharmakologie (FMP)

Title:
New chemoselective reactions for the site-specific posttranslational modification and cellular delivery of functional proteins

Place: Lilla hörsalen, KB3A9

Host: Christian Hedberg, Department of Chemistry


Abstract:
Our lab constantly aims to identify new bioorthogonal reactions for the synthesis and modification of functional peptides and proteins. We apply these highly selective organic reactions to study functional consequences of naturally occurring posttranslational protein modifications (PTMs) as well as to generate novel peptide- and protein-conjugates for pharmaceutical and medicinal applications.(1)

In the first part of this lecture, our recent efforts in the synthesis and analysis of naturally occurring labile PTMs including the synthesis of site-specifically phosphorylated Lys-peptides (pLys) using the Staudinger-phosphite reaction (2a) as well as phosphorylated Cys-peptides (pCys), (2b) will be presented.

In the second part of this presentation, I will focus on the cellular delivery of modified functional proteins. Thereby, we employ cyclic cell penetrating peptides (cCPPs) to transport a functional full length protein to the cytosol of living cells as recently demonstrated by the direct delivery of GFP-conjugates.(3) For protein modification we use a combined approach of intein expression, bioorthogonal reactions and recently developed enzymatic ligations, called Tub-tag labeling.4 This concept is finally applied to generate site-specifically labelled cell permeable nanobodies, i.e. small antigen binding proteins that remain active within the reductive milieu inside living cells, to interfere with intracellular targets.

References
(1.) D. Schumacher, C.P.R. Hackenberger, Curr. Opin. Chem. Biol. 2014, 22, 62-69.

(2.) a)J. Bertran-Vicente, R.A. Serwa, M. Schümann, P. Schmieder, E. Krause, C.P.R. Hackenberger, J. Am Chem. Soc. 2014, 136, 13622-13628;

(b) J. Bertran-Vicente, M. Penkert, O. Nieto, M. Schümann, P. Schmieder, E. Krause, C.P.R. Hackenberger, submitted.

(3.) N. Nischan, H.D. Herce, F. Natale, N. Bohlke, N. Budisa, M.C. Cardoso, C.P.R. Hackenberger, Angew. Chem. Int. Ed. 2015, 54, 1950-1953.

(4.) D. Schumacher, J. Helma, F.A. Mann, G. Pichler, F. Natale, E. Krause, M.C. Cardoso, C.P.R. Hackenberger, H. Leonhardt, Angew. Chem. Int. Ed. 2015, 54, 13787-13791.

Department of Chemistry

Thesis Defence

Michael Albers

Title: Synthesis and Investigation of bacterial effector molecules


Faculty Examiner: Christian Hackenberger, FMP Berlin.
Supervisor: Christian Hedberg.

Place: Lilla hörsalen, KBC

Friday 26 February, 10.00 (Note, change of time) Sal E04, NUS Trapphus R, 1 stairs down
Clinical Microbiology
Thesis defence
Marie Honn

 
Title:  Oxidative stressresponse hos Francisella tularensis
 
Faculty Examiner: Andres Melendez, Colleges of naoscale Science and
Engineering University of Albany, Sate University of New York
Supervisor Anders Sjöstedt