Month
Date: Monday, June 01, 2015 13:00 - 14:00
Categories: Seminar *

MIMS_UCMR Extra seminar

Speaker:
David A. Cisneros
IMP - Research Institute of Molecular Pathology
Vienna, Austria

Title:
"Bacterial secretion and eukaryotic chromosome segregation: Large molecular assemblies at work"

Place: Major Groove, Department of Molecular Biology, Bldg L, NUS Campus
Host: Bernt Eric Uhlin

Abstract:

Work is defined as the operation of a force in producing movement or other physical change. My research has mainly focused in understanding how large protein complexes interact structurally and biochemically to produce this kind of work.
I will first discuss the correlation of molecular dynamic simulations with biochemical data, which I used to study the intermediate steps of assembly of the type II secretion system. This system is a large multi-protein complex that secretes folded proteins from the periplasm of Gram-negative bacteria to the extracellular space.
I will then discuss the cohesin complex. In eukaryotic cells, this complex forms a ring with a diameter of ~50 nm that is thought to topologically entrap the two sister chromatids after DNA replication. During metaphase, the cohesin complex ensures sister chromatid bi-orientation, which allows their equal segregation. I am using Cas9 genome engineering and super-resolution microscopy to study its assembly before and after replication.