Department of Molecular Biology/MIMS
Extra Seminar
Speaker:
Himanshu Sharma
Indian Institute
of Technology Guwahati\, Assam\, India
Title:
Ribosom
e assembly defects abrogate initiation factor mediated inspection of start
codon
Host:
Jonas Barandun\, MIMS group leader and
SciLifeLab National Fellow\, Department of Molecular Biology
Room: Thymine\, Dept Molecular Biology\, NUS\, bldg 6K/L 2 stairs up
\nAbstract:
Faithful protein synthesis is ensured by ext
ensive quality control checkpoints using components of the translational m
achinery. However\, the implications of defective ribosome assembly on pro
tein synthesis and the associated translation quality control mechanisms i
n bacteria remain grossly unaddressed. In this study\, using E. coli as a
model organism we show that premature ribosomes evade scrutiny of cognate
start Codon by initiation factors during translation initiation. Resultant
ly the defective ribosomes make way into the translation cycle with severe
ly compromised decoding and protein synthesis capabilities. The enfeebled
binding of premature ribosomes to initiation factors also licenses the rap
id conversion of the 30S (pre) initiation complex to 70S initiation comple
x. Overall\, our work highlights that a mass balance deficit between prema
ture ribosomes and initiation factors steers the entry of premature riboso
mes into the translation cycle.
(Co-Author: B. Anand)
Department of Molecular Biology/MIMS
E
xtra Seminar
Speaker:
Himanshu Sharma
I
ndian Institute of Technology Guwahati\, Assam\, India
Title:
Ribosome assembly defects abrogate initiation factor mediated ins
pection of start codon
Host:
Jonas Barandun\, MIMS g
roup leader and SciLifeLab National Fellow\, Department of Molecular Biolo
gy
Room: Thymine\, Dept Molecular Biology\, NUS\, bldg 6K/L 2 stai rs up
\nAbstract:
Faithful protein synthesis i
s ensured by extensive quality control checkpoints using components of the
translational machinery. However\, the implications of defective ribosome
assembly on protein synthesis and the associated translation quality cont
rol mechanisms in bacteria remain grossly unaddressed. In this study\, usi
ng E. coli as a model organism we show that premature ribosomes evade scru
tiny of cognate start Codon by initiation factors during translation initi
ation. Resultantly the defective ribosomes make way into the translation c
ycle with severely compromised decoding and protein synthesis capabilities
. The enfeebled binding of premature ribosomes to initiation factors also
licenses the rapid conversion of the 30S (pre) initiation complex to 70S i
nitiation complex. Overall\, our work highlights that a mass balance defic
it between premature ribosomes and initiation factors steers the entry of
premature ribosomes into the translation cycle.
(Co-Author: B. Anand)
Department of Molecular Biology/MIMS
E
xtra Seminar
Magnus Ölander
Department of Ph
armacy\, Uppsala University
Title:
Proteomic and functio
nal analysis of
in vitro systems for studi
es of drug disposition in the human small intestine and liver
Place: Thymine\, Molecular Biology bldg. 6K/L (2 stairs up)
Host: Barbara Sixt\, MIMS group leader\, \; Dept of Molecular Bio logy
\n\;
DTSTART;TZID=Europe/Stockholm:20190529T133000 DTEND;TZID=Europe/Stockholm:20190529T143000 SUMMARY:Proteomic and functional analysis of in vitro systems for studies o f drug disposition in the human small intestine and liver URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/164-seminar/ 579-proteomic-and-functional-analysis-of-in-vitro-systems-for-studies-of-d rug-disposition-in-the-human-small-intestine-and-liver.html X-ALT-DESC;FMTTYPE=TEXT/HTML:Department of Molecular Biology/MIM
S
Extra Seminar
Magnus Ölander
Department of Pharmacy\, Uppsala University
Title:
Prot
eomic and functional analysis of
in vitro
systems for studies of drug disposition in the human small intestine and l
iver
Place: Thymine\, Molecular Biology bldg. 6K/L
(2 stairs up)
Host: Barbara Sixt\, MIMS group leader\, \; Dept of Molecular Biology
\n\;
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