'RNAIII of Staphylococcus aureus and its ro
le in cell wall homeostasis'
Place: room Thymine\, Department of Mole
cular Biology\, NUS Area\, bldg 6L
Host: Jörge
n Johansson
END:VEVENT
BEGIN:VEVENT
UID:193fc148b218dd878941c1c44f0efcd3http://www.ucmr.umu.se
DTSTAMP:20260624T134612Z
CATEGORIES:General
DESCRIPTION:Thesis Defence
\nJonas
Gripenland\, Department of Molecular Biology
\n'Reg
ulatory roles of two small RNAs in the human pathogen Listeria monocyt
ogenes and Evaluation of an alternative infection model'
\nPlace: Major Groove\, bldg. 6L\, NUS
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/strong>
\nOpponent: Thomas Geissmann\, Associate
Professor\, Université de Lyon\, Frankrike.
\nSupervisor: Jörgen Johansson\, Department of Molecular Biology
DTSTART;TZID=Europe/Stockholm:20120615T100000
DTEND;TZID=Europe/Stockholm:20120615T123000
SUMMARY:Thesis Defence - Jonas Gripenland: Regulatory roles of two small RN
As in the human pathogen Listeria monocytogeneses and Evaluation of an alt
ernative infection model
URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/165-general/
208-thesis-defence-jonas-gripenland-regulatory-roles-of-two-small-rnas-in-
the-human-pathogen-listeria-monocytogeneses-and-evaluation-of-an-alternati
ve-infection-model.html
X-ALT-DESC;FMTTYPE=TEXT/HTML:Thesis Defence
\n
Jonas Gripenland\, Department of Molecular Biology
\n'Regulatory roles of two small RNAs in the human pathogen Listeria monocytogenes and Evaluation of an alternative infection mo
del'
\nPlace: Major Groove\, bldg. 6L\, NU
S
\nOpponent: Thomas Geiss
mann\, Associate Professor\, Université de Lyon\, Frankrike.
\nSupervisor: Jörgen Johansson\, Department of Molecular Biology
END:VEVENT
BEGIN:VEVENT
UID:beb24b75e97a70ecec2b044fda7d8744http://www.ucmr.umu.se
DTSTAMP:20260624T134612Z
CATEGORIES:General
DESCRIPTION:KBC-Seminar
Kazuya Kikuc
hi\, Osaka University\,
Graduate School of Engineering\, WPI-Immunology Frontier Research Center\, Osaka\, Japan
'Design\, synthesis and biol
ogical application of in vivo imaging probes with tunable chemical switche
s'
Place: KB3B1\, Stora hörsalen\, KB
C
Host: Gunnar Öquist\, Department of Plant
Physiology
Abstract:
One of the
great challenges in the post-genome era is to clarify the biological sign
ificance of intracellular molecules directly in living cells. If we can i
mage a molecule in action\, it is possible to acquire biological informat
ion\, which is unavailable if we deal with cell homogenates. One possible
approach is to design and synthesize chemical probes that can convert bi
ological information to chemical output. Protein fluorescent labeling pro
vides an attractive approach to study the localization and function of pr
oteins in living cells. Recently\, a specific pair of a protein tag and i
ts ligand has been utilized to visualize a protein of interest (POI). In
this method\, a POI is fused with a protein tag and the tag is labeled wi
th the ligand connected to a fluorescent molecule. The advantage of this
protein labeling system is that a variety of fluorescent molecules are po
tentially available as labeling reagents\, and that the protein tag is co
nditionally labeled with its fluorescent ligand. I have designed a protei
n labeling system that allows fluorophores to be linked to POI. The prote
in tag (BL-tag) is a mutant class A ?-lactamase (TEM-1) modified to be co
valently bound to the designed specific labeling probes and the labeling
probes is consisted with a ?-lactam ring (ampicillin\, cephalosporin) att
ached to various fluorophores. A fluorogenetic labeling system can be des
igned using the unique property of cephalosporin\, which release leaving
group by subsequent reaction after opening the lactam ring. For further
sophisticated application\, multicolor imaging was done by adopting the c
olorful fluorophores.
DTSTART;TZID=Europe/Stockholm:20120615T133000
DTEND;TZID=Europe/Stockholm:20120615T133000
SUMMARY:Seminar - Kazuya Kikuchi: Design\, synthesis and biological applica
tion of in vivo imaging probes with tunablechemical switches
URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/165-general/
220-seminar-kazuya-kikuchi-design-synthesis-and-biological-application-of-
in-vivo-imaging-probes-with-tunablechemical-switches.html
X-ALT-DESC;FMTTYPE=TEXT/HTML:KBC
-Seminar
Kazuya Kikuchi\, O
saka University\, Graduate School of Engineering\, WPI-Immunology Frontier Research Center\, Osaka\, Japan
'Design\, s
ynthesis and biological application of in vivo imaging probes with tunable
chemical switches'
Place: KB3B1\, St
ora hörsalen\, KBC
Host: Gunnar Öquist\, Dep
artment of Plant Physiology
Abstract:
One of the great challenges in the post-genome era is to clarify the
biological significance of intracellular molecules directly in living c
ells. If we can image a molecule in action\, it is possible to acquire bi
ological information\, which is unavailable if we deal with cell homogena
tes. One possible approach is to design and synthesize chemical probes th
at can convert biological information to chemical output. Protein fluores
cent labeling provides an attractive approach to study the localization a
nd function of proteins in living cells. Recently\, a specific pair of a
protein tag and its ligand has been utilized to visualize a protein of in
terest (POI). In this method\, a POI is fused with a protein tag and the
tag is labeled with the ligand connected to a fluorescent molecule. The a
dvantage of this protein labeling system is that a variety of fluorescent
molecules are potentially available as labeling reagents\, and that the
protein tag is conditionally labeled with its fluorescent ligand. I have
designed a protein labeling system that allows fluorophores to be linked
to POI. The protein tag (BL-tag) is a mutant class A ?-lactamase (TEM-1)
modified to be covalently bound to the designed specific labeling probes
and the labeling probes is consisted with a ?-lactam ring (ampicillin\, c
ephalosporin) attached to various fluorophores. A fluorogenetic labeling
system can be designed using the unique property of cephalosporin\, which
release leaving group by subsequent reaction after opening the lactam ri
ng. For further sophisticated application\, multicolor imaging was done b
y adopting the colorful fluorophores.
END:VEVENT
BEGIN:VEVENT
UID:2e9d4c6ee738d559a88031088df22e38http://www.ucmr.umu.se
DTSTAMP:20260624T134612Z
CATEGORIES:Seminar
DESCRIPTION:Umeå Plant Science Centre Seminar
<
span style='font-size: 8pt\;'>Janne Lehtiö\, Karolinska Instit
ute\, Stockholm
'Defining human proteome us
ing high resolution peptide isoelectric focusing coupled to mass spectrome
try (HiRIEF-LC-MS) and the application of this method to improve cancer tr
eatment '
Place: Lilla
hörsalen\, KB3A9
Host: Anders Nordström\, Depa
rtment of Molecular Biology
DTSTART;TZID=Europe/Stockholm:20120615T150000
DTEND;TZID=Europe/Stockholm:20120615T160000
SUMMARY:Seminar - Janne Lehtiö: Defining human proteome using high resoluti
on peptide isoelectric focusing coupled to mass spectrometry (HiRIEF-LC-MS
) and the application of this method to improve cancer treatment
URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/164-seminar/
217-seminar-janne-lehtioe-defining-human-proteome-using-high-resolution-pe
ptide-isoelectric-focusing-coupled-to-mass-spectrometry-hirief-lc-ms-and-t
he-application-of-this-method-to-improve-cancer-treatment.html
X-ALT-DESC;FMTTYPE=TEXT/HTML:Umeå Plant Science Centre Seminar