UCMR-MIMS Seminar

Speaker:
Jay C. D. Hinton
Institute of Integrative Biology, University of Liverpool, UK,

Title:
Understanding the choreography of Salmonella transcription with RNA-seq

Place: Thymine, Department of Molecular Biology, 2nd floor
http://www.umu.se/english/about-umu/campus-maps?id=480

Host: Bernt Eric Uhlin & Mikael Rhen

Abstract:

Bacterial transcriptional networks typically consist of hundreds of transcription factors and thousands of promoters. However, the true complexity of transcription in a bacterial pathogen remains to be established. We devised a suite of 22 different environmental conditions that reflect the pathogenic lifestyle of Salmonella enterica serovar Typhimurium strain 4/74 during infection of a host, and used RNA-seq to generate a compendium of Salmonella gene expression. Individual in vitro conditions stimulated characteristic transcriptional signatures, and the suite of 22 conditions induced expression of 86% of all S. typhimurium genes 1. Recently, we compared these in vitro conditions with the intra-macrophage gene expression profiles of S. typhimurium 2.  All the data can be accessed at http://tinyurl.com/SalComMac. We hope that this database of environmentally-controlled expression of every transcriptional feature of S. typhimurium will be a useful resource for the bacterial research community, and the results explain the environmental signals that stimulate S. typhimurium promoters during infection.

We found that the transcription of the majority of the 3825 S. typhimurium promoters is environmentally-responsive. Our global approach has identified 280 small RNAs in S. typhimurium; these sRNAs were analysed in the context of chromosome localisation and Hfq-association.

I will discuss the environmental conditions that stimulate expression of the Salmonella pathogenicity islands, will present a comprehensive expression landscape of sRNAs and discuss our approach for defining the relative strength of S. typhimurium promoters during macrophage infection.

E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

References

1 Srikumar S, Kröger C, Hébrard M, Colgan A, Owen SV, Sivasankaran SK, Cameron ADS, Hokamp K, Hinton JCD: RNA-seq Brings New Insights to the Intra-Macrophage Transcriptome of Salmonella Typhimurium. PLOS Pathogens 2015, 11:e1005262.

2 Kröger C, Colgan A, Srikumar S, Handler K, Sivasankaran SK, Hammarlof DL, Canals R, Grissom JE, Conway T, Hokamp K, Hinton JC: An infection-relevant transcriptomic compendium for Salmonella enterica Serovar Typhimurium. Cell Host Microbe 2013, 14:683-695.

Department of Chemistry Seminar

Speaker:
Aymelt Itzen
Professor, Department Chemistry, Group of Proteinchemistry, TU München, Germany

Title:
Molecular mechanisms of the manipulation of small GTPases in the context of bacterial infections

Abstract:

Intracellular signaling processes of eukaryotic cells are strictly coordinated spatially and temporally. Here, G-proteins of the class of small GTPases play a key regulatory role since they function as molecular switches that stimulate or inhibit intracellular signal progression. Due to the central role of small GTPases in the maintenance of cell homeostasis and in the response to changing environmental conditions, these proteins are frequently targeted by bacterial pathogens. Bacteria manipulate the activities of small GTPases in order to manifest an infection. In this talk, I will discuss molecular mechanisms of the manipulation of small GTPases and other essential intracellular proteins in the context of bacterial infection strategies. In particular, I will focus on the consequences of eukaryotic protein modifications (e.g. adenylylation, phosphocholination, proteolysis) caused by bacterial enzymes and will discuss their application potential.

Host: Christian Hedberg, Dept of Chemistry

Place: KBC, Lilla hörsalen, KB3A9

Department of Clinical Microbiology
Thesis defence

Annasara Lenman

Title:
Adenovirus-host interactions: implications for tropism and therapy

Faculty Examiner: Lennart Svensson, Linköping University
Supervisor: Niklas Arnberg

Place:Lecture hall E04

(map:http://www.umu.se/english/about-umu/campus-maps?id=425)

Welcome to this extra UCMR – MIMS seminar!

Extra MIMS-UCMR seminar

Title:
"Virulence Strategies of Enterococci"

Speaker:
Dr. Kimberly Kline

Assistant Professor
Singapore Centre on Environmental Life Sciences
Engineering (SCELSE), School of Biological Sciences,
Nanyang Technological University, Singapore

Hosts: Fredrik Almqvist & Jörgen Johansson
Department of Chemistry/Department of Molecular biology

Place: Dept of Molecular Biology, Seminar Room Thymine (2 stairs up)

More information about Dr. Kline:
http://kimberlyklinelab.com/about/index.html

How to find Thymine:
http://www.umu.se/om-universitetet/kartor/?id=480

In connection with the meeting of the MIMS-UCMR Board in Umeå we will organise a Mini-Symposium.

Room: Triple Helix, University Liaison Building, Level 3

Chairperson:
Anna Arnqvist, Department of Medical Chemistry and Biophysics
Niklas Arnberg, Department of Clinical Microbiology

Programme:

08.30-08.50 
Anna Linusson, Group leader, Department of Chemistry, UmU
Discovery of selective inhibitors targeting acetylcholinesterase 1 from disease-transmitting mosquitoes

08.50-09.10 
Mikael Rhen, Karolinska Institute. UCMR/MIMS Guest professor
Alternative approaches for studying salmonellosis

09.10-09.30  
Nelson Gekara, MIMS Group Leader, Molecular Biology, UmU
Role of DNA repair proteins in Innate Immune Regulation

09.30–09.50
Anna Överby, MIMS Group Leader, Clinical Microbiology, UmU
Tick-borne encephalitis virus and the antiviral interferon system  

9.50–10.10    Coffe

10.10-10.30  
Vasili Hauryliuk, MIMS Group Leader, Molecular Biology, UmU
The role of ribosome-associated stress factors in antibiotic tolerance and resistance

10.30–10.50 
Andrea Puhar, MIMS Group Leader, Molecular Biology, UmU
Regulation of inflammation by the danger signal ATP in the gut

10.50–11.10
Magnus Wolf-Watz, Group leader, Department of Chemistry, UmU
Protein dynamics in T3SS mediated effector secretion

11.10- 11.30
Jörgen Johansson, MIMS Group Leader, Molecular Biology, UmU
Disarming bacterial pathogens

Interested people are welcome to listen to the symposium. Due to limitation of time and space we will organise light lunch for the participating speakers, board members and members of the MIMS/UCMR management only, outside of the lecture room, after the symposium.

Contact persons:
for the scientific programme: Åke Forsberg
for the organisation: Eva-Maria Diehl