BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//http://www.ucmr.umu.se//NONSGML kigkonsult.se iCalcreator 2.29.29
 //
CALSCALE:GREGORIAN
METHOD:PUBLISH
UID:2ca834ba-ce42-499c-be24-8e355e6073a4
X-WR-CALNAME:JCal Pro Calendar
X-WR-CALDESC:Your online events calendar
X-WR-TIMEZONE:Europe/Stockholm
BEGIN:VTIMEZONE
TZID:Europe/Stockholm
BEGIN:STANDARD
TZNAME:CET
DTSTART:20251026T030000
TZOFFSETFROM:+0200
TZOFFSETTO:+0100
RDATE:20261025T030000
RDATE:20271031T030000
END:STANDARD
BEGIN:DAYLIGHT
TZNAME:CEST
DTSTART:20260329T020000
TZOFFSETFROM:+0100
TZOFFSETTO:+0200
RDATE:20270328T020000
END:DAYLIGHT
END:VTIMEZONE
BEGIN:VEVENT
UID:beb24b75e97a70ecec2b044fda7d8744http://www.ucmr.umu.se
DTSTAMP:20260617T173818Z
CATEGORIES:General
DESCRIPTION:<span style='font-size: x-small\;'><strong>KBC-Seminar</strong>
 <br /><br /></span><strong><span style='font-size: x-small\;'>Kazuya Kikuc
 hi</span></strong><span style='font-size: x-small\;'>\, Osaka University\,
  Graduate School of Engineering\, </span><span style='font-size: x-small\;
 '>WPI-Immunology Frontier Research Center\, Osaka\, Japan</span><br /><spa
 n style='font-size: x-small\;'><br /><strong> 'Design\, synthesis and biol
 ogical application of in vivo imaging probes with tunable chemical switche
 s'</strong><br /><br /><strong>Place:</strong> KB3B1\, Stora hörsalen\, KB
 C<br /> <br /> <strong>Host:</strong> Gunnar Öquist\, Department of Plant 
 Physiology<br /> <br /><strong>Abstract: </strong></span><br />One of the 
 great challenges in the post-genome era is to clarify the  biological sign
 ificance of intracellular molecules directly in living  cells. If we can i
 mage a molecule in action\, it is possible to acquire  biological informat
 ion\, which is unavailable if we deal with cell  homogenates. One possible
  approach is to design and synthesize chemical  probes that can convert bi
 ological information to chemical output.  Protein fluorescent labeling pro
 vides an attractive approach to study  the localization and function of pr
 oteins in living cells. Recently\, a  specific pair of a protein tag and i
 ts ligand has been utilized to  visualize a protein of interest (POI). In 
 this method\, a POI is fused  with a protein tag and the tag is labeled wi
 th the ligand connected to a  fluorescent molecule. The advantage of this 
 protein labeling system is  that a variety of fluorescent molecules are po
 tentially available as  labeling reagents\, and that the protein tag is co
 nditionally labeled  with its fluorescent ligand. I have designed a protei
 n labeling system  that allows fluorophores to be linked to POI. The prote
 in tag (BL-tag)  is a mutant class A ?-lactamase (TEM-1) modified to be co
 valently bound  to the designed specific labeling probes and the labeling 
 probes is  consisted with a ?-lactam ring (ampicillin\, cephalosporin) att
 ached to  various fluorophores. A fluorogenetic labeling system can be des
 igned  using the unique property of cephalosporin\, which release leaving 
 group  by subsequent reaction after opening the lactam ring. For further  
 sophisticated application\, multicolor imaging was done by adopting the  c
 olorful fluorophores.
DTSTART;TZID=Europe/Stockholm:20120615T133000
DTEND;TZID=Europe/Stockholm:20120615T133000
SUMMARY:Seminar - Kazuya Kikuchi: Design\, synthesis and biological applica
 tion of in vivo imaging probes with tunablechemical switches
URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/165-general/
 220-seminar-kazuya-kikuchi-design-synthesis-and-biological-application-of-
 in-vivo-imaging-probes-with-tunablechemical-switches.html
X-ALT-DESC;FMTTYPE=TEXT/HTML:<span style='font-size: x-small\;'><strong>KBC
 -Seminar</strong><br /><br /></span><strong><span style='font-size: x-smal
 l\;'>Kazuya Kikuchi</span></strong><span style='font-size: x-small\;'>\, O
 saka University\, Graduate School of Engineering\, </span><span style='fon
 t-size: x-small\;'>WPI-Immunology Frontier Research Center\, Osaka\, Japan
 </span><br /><span style='font-size: x-small\;'><br /><strong> 'Design\, s
 ynthesis and biological application of in vivo imaging probes with tunable
  chemical switches'</strong><br /><br /><strong>Place:</strong> KB3B1\, St
 ora hörsalen\, KBC<br /> <br /> <strong>Host:</strong> Gunnar Öquist\, Dep
 artment of Plant Physiology<br /> <br /><strong>Abstract: </strong></span>
 <br />One of the great challenges in the post-genome era is to clarify the
   biological significance of intracellular molecules directly in living  c
 ells. If we can image a molecule in action\, it is possible to acquire  bi
 ological information\, which is unavailable if we deal with cell  homogena
 tes. One possible approach is to design and synthesize chemical  probes th
 at can convert biological information to chemical output.  Protein fluores
 cent labeling provides an attractive approach to study  the localization a
 nd function of proteins in living cells. Recently\, a  specific pair of a 
 protein tag and its ligand has been utilized to  visualize a protein of in
 terest (POI). In this method\, a POI is fused  with a protein tag and the 
 tag is labeled with the ligand connected to a  fluorescent molecule. The a
 dvantage of this protein labeling system is  that a variety of fluorescent
  molecules are potentially available as  labeling reagents\, and that the 
 protein tag is conditionally labeled  with its fluorescent ligand. I have 
 designed a protein labeling system  that allows fluorophores to be linked 
 to POI. The protein tag (BL-tag)  is a mutant class A ?-lactamase (TEM-1) 
 modified to be covalently bound  to the designed specific labeling probes 
 and the labeling probes is  consisted with a ?-lactam ring (ampicillin\, c
 ephalosporin) attached to  various fluorophores. A fluorogenetic labeling 
 system can be designed  using the unique property of cephalosporin\, which
  release leaving group  by subsequent reaction after opening the lactam ri
 ng. For further  sophisticated application\, multicolor imaging was done b
 y adopting the  colorful fluorophores.
END:VEVENT
END:VCALENDAR