UCMR-MIMS Seminar
\nSpeaker:
Jay C. D. Hi
nton
Institute of Integrative Biology\, University of Liverp
ool\, UK\,
Title:
Understanding the choreography of S
almonella transcription with RNA-seq
Place: Thymine\,
Department of Molecular Biology\, 2nd floor
http://www.umu.se/english/about-um
u/campus-maps?id=480
Host: Bernt Eric Uhlin &\; Mikael Rhen
\nAbstract:
\nBacterial transcriptional netw orks typically consist of hundreds of transcription factors and thousands of promoters. However\, the true complexity of transcription in a bacteria l pathogen remains to be established. We devised a suite of 22 different e nvironmental conditions that reflect the pathogenic lifestyle of Salmo nella enterica serovar Typhimurium strain 4/74 during infection of a host\, and used RNA-seq to generate a compendium of Salmonella gene expres sion. Individual in vitro conditions stimulated characteristic tr anscriptional signatures\, and the suite of 22 conditions induced expressi on of 86% of all S. typhimurium genes 1. Recently\, we compared t hese in vitro conditions with the intra-macrophage gene expressio n profiles of S. typhimurium 2. \;All the data can be access ed at http://tinyurl.com/SalComMac< /a>. We hope that this database of environmentally-controlled expression o f every transcriptional feature of S. typhimurium will be a usefu l resource for the bacterial research community\, and the results explain the environmental signals that stimulate S. typhimurium promoters during infection.
\nWe found that the transcription of the majority of the 3825 S. typhimurium promoters is environmentally-responsi ve. Our global approach has identified 280 small RNAs in S. typhimuriu m\; these sRNAs were analysed in the context of chromosome localisati on and Hfq-association.
\nI will discuss the environmental condition s that stimulate expression of the Salmonella pathogenicity islands\, will present a comprehensive expression landscape of sRNAs and discuss our app roach for defining the relative strength of S. typhimurium promot ers during macrophage infection.
\nE-mail: This email address is being protected from spambot s. You need JavaScript enabled to view it.
\n\;
\nReferences
\n1 Srikumar S\, Kr ö\;ger C\, Hé\;brard M\, Colgan A\, Owen SV\, Sivasankaran SK\, Cameron ADS\, Hokamp K\, Hinton JCD: RNA-seq Brings New Insights to the In tra-Macrophage Transcriptome of Salmonella Typhimurium. PLOS Pathogens 201 5\, 11:e1005262.
\n2 Krö\;ger C\, Colgan A\, Srikumar S\, Handle r K\, Sivasankaran SK\, Hammarlof DL\, Canals R\, Grissom JE\, Conway T\, Hokamp K\, Hinton JC: An infection-relevant transcriptomic compendium for Salmonella enterica Serovar Typhimurium. Cell Host Microbe 2013\, 14:683-6 95.
DTSTART;TZID=Europe/Stockholm:20160512T150000 DTEND;TZID=Europe/Stockholm:20160512T160000 SUMMARY:Seminar - Jay C.D. Hinton: Understanding the choreography of Salmon ella transcription with RNA-seq URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/164-seminar/ 417-seminar-jay-c-d-hinton-understanding-the-choreography-of-salmonella-tr anscription-with-rna-seq.html X-ALT-DESC;FMTTYPE=TEXT/HTML:UCMR-MIMS Seminar
\nSpeaker:
Institute of Integrative Biology\, Uni
versity of Liverpool\, UK\,
Title:
Understanding the
choreography of Salmonella transcription with RNA-seq
Place: Thymine\, Department of Molecular Biology\, 2nd floor
http://www.umu.se
/english/about-umu/campus-maps?id=480
Host: Bernt Eric Uhlin & amp\; Mikael Rhen
\nAbstract:
\nBacterial tra nscriptional networks typically consist of hundreds of transcription facto rs and thousands of promoters. However\, the true complexity of transcript ion in a bacterial pathogen remains to be established. We devised a suite of 22 different environmental conditions that reflect the pathogenic lifes tyle of Salmonella enterica serovar Typhimurium strain 4/74 durin g infection of a host\, and used RNA-seq to generate a compendium of Salmo nella gene expression. Individual in vitro conditions stimulated characteristic transcriptional signatures\, and the suite of 22 conditions induced expression of 86% of all S. typhimurium genes 1. Recentl y\, we compared these in vitro conditions with the intra-macropha ge gene expression profiles of S. typhimurium 2. \;All the d ata can be accessed at http://tinyu rl.com/SalComMac. We hope that this database of environmentally-contro lled expression of every transcriptional feature of S. typhimurium will be a useful resource for the bacterial research community\, and the results explain the environmental signals that stimulate S. typhimuri um promoters during infection.
\nWe found that the transcriptio n of the majority of the 3825 S. typhimurium promoters is environ mentally-responsive. Our global approach has identified 280 small RNAs in S. typhimurium\; these sRNAs were analysed in the context of chro mosome localisation and Hfq-association.
\nI will discuss the enviro nmental conditions that stimulate expression of the Salmonella pathogenici ty islands\, will present a comprehensive expression landscape of sRNAs an d discuss our approach for defining the relative strength of S. typhim urium promoters during macrophage infection.
\nE-mail: This email address is being prote cted from spambots. You need JavaScript enabled to view it.
\n\;
\nReferences
\n1 Srikumar S\, Krö\;ger C\, Hé\;brard M\, Colgan A\, Owen SV\, S ivasankaran SK\, Cameron ADS\, Hokamp K\, Hinton JCD: RNA-seq Brings New I nsights to the Intra-Macrophage Transcriptome of Salmonella Typhimurium. P LOS Pathogens 2015\, 11:e1005262.
\n2 Krö\;ger C\, Colgan A\, Sr ikumar S\, Handler K\, Sivasankaran SK\, Hammarlof DL\, Canals R\, Grissom JE\, Conway T\, Hokamp K\, Hinton JC: An infection-relevant transcriptomi c compendium for Salmonella enterica Serovar Typhimurium. Cell Host Microb e 2013\, 14:683-695.
END:VEVENT END:VCALENDAR