Next generation tools for studying UPEC pathogenesis
\nSpeaker: MD/PhD Swaine Chen\, Singapore NRF Fellow\, Nat ional University of Singapore and Genome Institute of Singapore\, Singapor e.
\nTitle: 'Next generation tools for studying UPEC pathoge nesis'
\nRoom: Major Groove
\nHosts: Fredrik Almqvist and Sven Bergström \;
\nAbstract: Urinary tract infections (UTI s) are extremely common infections affecting half of all women and contrib uting greatly to antibiotic prescriptions and therefore bacterial antibiot ic resistance. Most UTIs are caused by uropathogenic Escherichia coli (UPE C). The study of how UPEC cause UTIs is greatly facilitated by many geneti c and molecular tools available for E. coli in general. However\, these to ols are usually developed in and for cloning or lab-adapted strains of E. coli\, and they are sometimes not usable or not efficient in disease-causi ng clinical isolates such as UPEC.
\nMy lab has developed two new to ols with clinical strains in mind to improve our ability to study UTI. The first improves our ability to manipulate the UPEC chromosome. We have dev eloped a general and modular negative selection (counterselection) system that functions without optimization in multiple clinical isolates of E. co li and Salmonella. Furthermore\, this system functions up to 1000x better than all other reported systems in lab strains of E. coli. This system now enables the convenient creation of definitive genetic constructs directly in UPEC.
\nThe second project improves our ability to detect UPEC d uring infection. We have designed a new GFP protein which we term vGFP tha t demonstrates a 30-50% improvement in bulk brightness while simultaneousl y enabling rational control of dimerization state. vGFP is therefore a bet ter chromosomal reporter than other GFP variants and should improve our de tection sensitivity of UPEC\, which is paramount for tracking UPEC as they move through minor niches during infection.
\nTogether these tools improve our ability to translate our mouse studies into human disease. I w ill conclude with our plans for performing niche-specific\, single-cell re solved\, simultaneous host and pathogen transcriptional profiling in direc t UTI samples. In the future\, this will hopefully allow us to do a full g enomic translation of our lab studies into knowledge about human disease.< /p> DTSTART;TZID=Europe/Stockholm:20141030T150000 DTEND;TZID=Europe/Stockholm:20141030T160000 SUMMARY:Seminar - Swaine Chen: Next generation tools for studying UPEC path ogenesis URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/164-seminar/ 368-seminar-swaine-chen-next-generation-tools-for-studying-upec-pathogenes is.html X-ALT-DESC;FMTTYPE=TEXT/HTML:
Next generation tools for studying UPEC pathogenesis
\nSpeaker: MD/PhD Swaine Chen\, Singapore NRF Fellow\, National University of Singapore and Genome Institute of Sin gapore\, Singapore.
\nTitle: 'Next generation tools for stud ying UPEC pathogenesis'
\nRoom: Major Groove
\nHosts: Fredrik Almqvist and Sven Bergström \;
\nAbstract: Urinary trac t infections (UTIs) are extremely common infections affecting half of all women and contributing greatly to antibiotic prescriptions and therefore b acterial antibiotic resistance. Most UTIs are caused by uropathogenic Esch erichia coli (UPEC). The study of how UPEC cause UTIs is greatly facilitat ed by many genetic and molecular tools available for E. coli in general. H owever\, these tools are usually developed in and for cloning or lab-adapt ed strains of E. coli\, and they are sometimes not usable or not efficient in disease-causing clinical isolates such as UPEC.
\nMy lab has dev eloped two new tools with clinical strains in mind to improve our ability to study UTI. The first improves our ability to manipulate the UPEC chromo some. We have developed a general and modular negative selection (counters election) system that functions without optimization in multiple clinical isolates of E. coli and Salmonella. Furthermore\, this system functions up to 1000x better than all other reported systems in lab strains of E. coli . This system now enables the convenient creation of definitive genetic co nstructs directly in UPEC.
\nThe second project improves our ability to detect UPEC during infection. We have designed a new GFP protein which we term vGFP that demonstrates a 30-50% improvement in bulk brightness wh ile simultaneously enabling rational control of dimerization state. vGFP i s therefore a better chromosomal reporter than other GFP variants and shou ld improve our detection sensitivity of UPEC\, which is paramount for trac king UPEC as they move through minor niches during infection.
\nToge ther these tools improve our ability to translate our mouse studies into h uman disease. I will conclude with our plans for performing niche-specific \, single-cell resolved\, simultaneous host and pathogen transcriptional p rofiling in direct UTI samples. In the future\, this will hopefully allow us to do a full genomic translation of our lab studies into knowledge abou t human disease.
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