UCMR-MIMS Seminar
\nSpeaker:
Jay C. D. Hi
nton
Institute of Integrative Biology\, University of Liverp
ool\, UK\,
Title:
Understanding the choreography of S
almonella transcription with RNA-seq
Place: Thymine\,
Department of Molecular Biology\, 2nd floor
http://www.umu.se/english/about-um
u/campus-maps?id=480
Host: Bernt Eric Uhlin &\; Mikael Rhen
\nAbstract:
\nBacterial transcriptional netw orks typically consist of hundreds of transcription factors and thousands of promoters. However\, the true complexity of transcription in a bacteria l pathogen remains to be established. We devised a suite of 22 different e nvironmental conditions that reflect the pathogenic lifestyle of Salmo nella enterica serovar Typhimurium strain 4/74 during infection of a host\, and used RNA-seq to generate a compendium of Salmonella gene expres sion. Individual in vitro conditions stimulated characteristic tr anscriptional signatures\, and the suite of 22 conditions induced expressi on of 86% of all S. typhimurium genes 1. Recently\, we compared t hese in vitro conditions with the intra-macrophage gene expressio n profiles of S. typhimurium 2. \;All the data can be access ed at http://tinyurl.com/SalComMac< /a>. We hope that this database of environmentally-controlled expression o f every transcriptional feature of S. typhimurium will be a usefu l resource for the bacterial research community\, and the results explain the environmental signals that stimulate S. typhimurium promoters during infection.
\nWe found that the transcription of the majority of the 3825 S. typhimurium promoters is environmentally-responsi ve. Our global approach has identified 280 small RNAs in S. typhimuriu m\; these sRNAs were analysed in the context of chromosome localisati on and Hfq-association.
\nI will discuss the environmental condition s that stimulate expression of the Salmonella pathogenicity islands\, will present a comprehensive expression landscape of sRNAs and discuss our app roach for defining the relative strength of S. typhimurium promot ers during macrophage infection.
\nE-mail: This email address is being protected from spambot s. You need JavaScript enabled to view it.
\n\;
\nReferences
\n1 Srikumar S\, Kr ö\;ger C\, Hé\;brard M\, Colgan A\, Owen SV\, Sivasankaran SK\, Cameron ADS\, Hokamp K\, Hinton JCD: RNA-seq Brings New Insights to the In tra-Macrophage Transcriptome of Salmonella Typhimurium. PLOS Pathogens 201 5\, 11:e1005262.
\n2 Krö\;ger C\, Colgan A\, Srikumar S\, Handle r K\, Sivasankaran SK\, Hammarlof DL\, Canals R\, Grissom JE\, Conway T\, Hokamp K\, Hinton JC: An infection-relevant transcriptomic compendium for Salmonella enterica Serovar Typhimurium. Cell Host Microbe 2013\, 14:683-6 95.
DTSTART;TZID=Europe/Stockholm:20160512T150000 DTEND;TZID=Europe/Stockholm:20160512T160000 SUMMARY:Seminar - Jay C.D. Hinton: Understanding the choreography of Salmon ella transcription with RNA-seq URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/164-seminar/ 417-seminar-jay-c-d-hinton-understanding-the-choreography-of-salmonella-tr anscription-with-rna-seq.html X-ALT-DESC;FMTTYPE=TEXT/HTML:UCMR-MIMS Seminar
\nSpeaker:
Institute of Integrative Biology\, Uni
versity of Liverpool\, UK\,
Title:
Understanding the
choreography of Salmonella transcription with RNA-seq
Place: Thymine\, Department of Molecular Biology\, 2nd floor
http://www.umu.se
/english/about-umu/campus-maps?id=480
Host: Bernt Eric Uhlin & amp\; Mikael Rhen
\nAbstract:
\nBacterial tra nscriptional networks typically consist of hundreds of transcription facto rs and thousands of promoters. However\, the true complexity of transcript ion in a bacterial pathogen remains to be established. We devised a suite of 22 different environmental conditions that reflect the pathogenic lifes tyle of Salmonella enterica serovar Typhimurium strain 4/74 durin g infection of a host\, and used RNA-seq to generate a compendium of Salmo nella gene expression. Individual in vitro conditions stimulated characteristic transcriptional signatures\, and the suite of 22 conditions induced expression of 86% of all S. typhimurium genes 1. Recentl y\, we compared these in vitro conditions with the intra-macropha ge gene expression profiles of S. typhimurium 2. \;All the d ata can be accessed at http://tinyu rl.com/SalComMac. We hope that this database of environmentally-contro lled expression of every transcriptional feature of S. typhimurium will be a useful resource for the bacterial research community\, and the results explain the environmental signals that stimulate S. typhimuri um promoters during infection.
\nWe found that the transcriptio n of the majority of the 3825 S. typhimurium promoters is environ mentally-responsive. Our global approach has identified 280 small RNAs in S. typhimurium\; these sRNAs were analysed in the context of chro mosome localisation and Hfq-association.
\nI will discuss the enviro nmental conditions that stimulate expression of the Salmonella pathogenici ty islands\, will present a comprehensive expression landscape of sRNAs an d discuss our approach for defining the relative strength of S. typhim urium promoters during macrophage infection.
\nE-mail: This email address is being prote cted from spambots. You need JavaScript enabled to view it.
\n\;
\nReferences
\n1 Srikumar S\, Krö\;ger C\, Hé\;brard M\, Colgan A\, Owen SV\, S ivasankaran SK\, Cameron ADS\, Hokamp K\, Hinton JCD: RNA-seq Brings New I nsights to the Intra-Macrophage Transcriptome of Salmonella Typhimurium. P LOS Pathogens 2015\, 11:e1005262.
\n2 Krö\;ger C\, Colgan A\, Sr ikumar S\, Handler K\, Sivasankaran SK\, Hammarlof DL\, Canals R\, Grissom JE\, Conway T\, Hokamp K\, Hinton JC: An infection-relevant transcriptomi c compendium for Salmonella enterica Serovar Typhimurium. Cell Host Microb e 2013\, 14:683-695.
END:VEVENT BEGIN:VEVENT UID:20160525T100000UTC-15698e@http://www.ucmr.umu.se/ DTSTAMP:20260515T024950Z CATEGORIES:Seminar DESCRIPTION:Department of Chemistry Seminar
\nSpeaker:
Professor\, Department Chemistry\, Group of
Proteinchemistry\, TU Mü\;nchen\, Germany
Title:
Abstract:
\nIntracellular signaling processes of eukaryotic cells are strictly coordinated spatially and temporally. Here\, G-proteins of the class of small GTPases play a ke y regulatory role since they function as molecular switches that stimulate or inhibit intracellular signal progression. Due to the central role of s mall GTPases in the maintenance of cell homeostasis and in the response to changing environmental conditions\, these proteins are frequently targete d by bacterial pathogens. Bacteria manipulate the activities of small GTPa ses in order to manifest an infection. In this talk\, I will discuss molec ular mechanisms of the manipulation of small GTPases and other essential i ntracellular proteins in the context of bacterial infection strategies. In particular\, I will focus on the consequences of eukaryotic protein modif ications (e.g. adenylylation\, phosphocholination\, proteolysis) caused by bacterial enzymes and will discuss their application potential.
\n& nbsp\;
\nHost: Christian Hedberg\, Dept of Chemistry
\n\;
\nPlace: KBC\, Lilla hö\;rsalen\, KB3A9
DTSTART;TZID=Europe/Stockholm:20160525T120000 DTEND;TZID=Europe/Stockholm:20160525T130000 SUMMARY:Seminar - Aymelt Itzen: Molecular mechanisms of the manipulation of small GTPases in the context of bacterial infections URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/164-seminar/ 421-seminar-aymelt-itzen-molecular-mechanisms-of-the-manipulation-of-small -gtpases-in-the-context-of-bacterial-infections.html X-ALT-DESC;FMTTYPE=TEXT/HTML:Department of Chemistry Seminar
\nSpe
aker:
Aymelt Itzen
Professor\, Department Che
mistry\, Group of Proteinchemistry\, TU Mü\;nchen\, Germany
Ti
tle:
Molecular mechanisms of the manipulation of small GTPas
es in the context of bacterial infections
Abstract:
\nIntracellular signaling processes of eukaryotic cells are strictly coor dinated spatially and temporally. Here\, G-proteins of the class of small GTPases play a key regulatory role since they function as molecular switch es that stimulate or inhibit intracellular signal progression. Due to the central role of small GTPases in the maintenance of cell homeostasis and i n the response to changing environmental conditions\, these proteins are f requently targeted by bacterial pathogens. Bacteria manipulate the activit ies of small GTPases in order to manifest an infection. In this talk\, I w ill discuss molecular mechanisms of the manipulation of small GTPases and other essential intracellular proteins in the context of bacterial infecti on strategies. In particular\, I will focus on the consequences of eukaryo tic protein modifications (e.g. adenylylation\, phosphocholination\, prote olysis) caused by bacterial enzymes and will discuss their application pot ential.
\n\;
\nHost: Christian Hedberg\, Dept of Chemistry
\n\;
\nPlace: KBC\, Lilla hö\;rsalen\, KB3A9
END:VEVENT BEGIN:VEVENT UID:20160527T070000UTC-3b43d2@http://www.ucmr.umu.se/ DTSTAMP:20260515T024950Z CATEGORIES:Events DESCRIPTION:Department of Clinical Microbiology
Thesis defence
Annasara Lenman
\nTitle:
Adenovir
us-host interactions: implications for tropism and therapy
Place:Lecture hall E04
(map:http://www.u
mu.se/english/about-umu/campus-maps?id=425)
Department of Clinical Microbiology
Th
esis defence
Annasara Lenman
\nTitle:
Adenovirus-host interactions: implications for tropism and therapy
Faculty Examiner: Lennart Svensson\, Linkö\;ping Uni
versity
Supervisor: Niklas Arnberg
Place:Lecture hall E04
(map:http://www.umu.se/english/about-umu/campus-maps?id=425)
Welcome to this extra UCMR &ndash\; MIMS seminar!
\nEx tra MIMS-UCMR seminar
\nTitle:
'Virulence Strategies o
f Enterococci'
Speaker:
Dr. Kimberly Kline
Assistant Professor
Singapore Centre on Environmental
Life Sciences
Engineering (SCELSE)\, School of Biological Sciences\,
Nanyang Technological University\, Singapore
H
osts: Fredrik Almqvist &\; Jö\;rgen Johansson
Departm
ent of Chemistry/Department of Molecular biology
Place: Dept of Molecular Biology\, Seminar Room Thymine (2 stairs up)
\nMore information about Dr. Kline:
http://kimberlyklinelab.com/abou
t/index.html
How to find Thymine:
http://www.umu.se/om
-universitetet/kartor/?id=480
\;
DTSTART;TZID=Europe/Stockholm:20160527T093000 DTEND;TZID=Europe/Stockholm:20160527T103000 SUMMARY:UCMR-MIMS Extra Seminar - Kimberly Kline: Virulence Strategies of E nterococci URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/164-seminar/ 420-ucmr-mims-extra-seminar-kimberly-kline-virulence-strategies-of-enteroc occi.html X-ALT-DESC;FMTTYPE=TEXT/HTML:Welcome to this extra UCMR &ndash\; MIMS se minar!
\nExtra MIMS-UCMR seminar
\nTitle:
'Virul
ence Strategies of Enterococci'
Speaker:
Dr.
Kimberly Kline
Assistant Professor
Singapore Centre
on Environmental Life Sciences
Engineering (SCELSE)\, School of Biol
ogical Sciences\,
Nanyang Technological University\, Singapore
Place: Dept of Molecular Biology\, Seminar Room Thymine ( 2 stairs up)
\nMore information about Dr. Kline:
http://kimberlyk
linelab.com/about/index.html
How to find Thymine:
http
://www.umu.se/om-universitetet/kartor/?id=480
\;
END:VEVENT BEGIN:VEVENT UID:20160531T063000UTC-a1eae5@http://www.ucmr.umu.se/ DTSTAMP:20260515T024950Z CATEGORIES:Events DESCRIPTION:In connection with the meeting of the MIMS-UCMR Board in Ume å\; we will organise a Mini-Symposium.
\nRoom: Triple Helix\, University Liaison Building\, Level 3
\nChai
rperson:
Anna Arnqvist\, Department of Medical Chemistry an
d Biophysics
Niklas Arnberg\, Department of Clinical Microbiology
Programme:
\n08.30-08.50 \;
Anna Lin
usson\, Group leader\, Department of Chemistry\, UmU
Disc
overy of selective inhibitors targeting acetylcholinesterase 1 from diseas
e-transmitting mosquitoes
08.50-09.10 \;
M
ikael Rhen\, Karolinska Institute. UCMR/MIMS Guest professor
09.10-09.30 \;
Nelson Gekara\, MIMS Group Leader\, Molecular
Biology\, UmU
Role of DNA repair proteins in Innate Immu
ne Regulation
09.30&ndash\;09.50
Anna Ö\;v
erby\, MIMS Group Leader\, Clinical Microbiology\, UmU
Ti
ck-borne encephalitis virus and the antiviral interferon system \
; \;
9.50&ndash\;10.10 \; \; Coffe
\n1
0.10-10.30 \; \;
Vasili Hauryliuk\, MIMS Group Leader\, Mole
cular Biology\, UmU
The role of ribosome-associated stres
s factors in antibiotic tolerance and resistance
10.
30&ndash\;10.50 \;
Andrea Puhar\, MIMS Group Leader\, Molecular
Biology\, UmU
Regulation of inflammation by the danger si
gnal ATP in the gut
10.50&ndash\;11.10
Magnus
Wolf-Watz\, Group leader\, Department of Chemistry\, UmU
Protein dynamics in T3SS mediated effector secretion
\;
\nInterested people are welcome to listen to the symposium. Due to limitation of time and space we will organise light lunc h for the participating speakers\, board members and members of the MIMS/U CMR management only\, outside of the lecture room\, after the symposium.\n
Contact persons:
for the scientific programme: Å\;ke Fors
berg
for the organisation: Eva-Maria Diehl
In connection with the meeting of the MIMS- UCMR Board in Umeå\; we will organise a Mini-Symposium.
\nChairperson:
Anna Arnqvist\, Department of Med
ical Chemistry and Biophysics
Niklas Arnberg\, Department of Clinical
Microbiology
Programme:
\n08.30-08.50
\;
Anna Linusson\, Group leader\, Department of Chemistry\, UmU
Discovery of selective inhibitors targeting acetylcholinester
ase 1 from disease-transmitting mosquitoes
08.50-09.
10 \;
Mikael Rhen\, Karolinska Institute. UCMR/MIMS Guest profes
sor
Alternative approaches for studying salmonellosis
09.10-09.30 \;
Nelson Gekara\, MIMS Group L
eader\, Molecular Biology\, UmU
Role of DNA repair protei
ns in Innate Immune Regulation
09.30&ndash\;09.50 Anna Ö\;verby\, MIMS Group Leader\, Clinical Microbiology\, UmU
Tick-borne encephalitis virus and the antiviral interferon
system \; \;
9.50&ndash\;10.10 \; \ ; Coffe
\n10.10-10.30 \; \;
Vasili Hauryliuk\, MIMS Gr
oup Leader\, Molecular Biology\, UmU
The role of ribosome
-associated stress factors in antibiotic tolerance and resistance
10.30&ndash\;10.50 \;
Andrea Puhar\, MIMS Group Le
ader\, Molecular Biology\, UmU
Regulation of inflammation
by the danger signal ATP in the gut
10.50&ndash\;11
.10
Magnus Wolf-Watz\, Group leader\, Department of Chemistry\, UmU
Protein dynamics in T3SS mediated effector secretion
11.10- 11.30
Jö\;rgen Johansson\, MIMS Group
Leader\, Molecular Biology\, UmU
Disarming bacterial path
ogens
\;
\nInterested people are welcome to listen to the symposium. Due to limitation of time and space we will or ganise light lunch for the participating speakers\, board members and memb ers of the MIMS/UCMR management only\, outside of the lecture room\, after the symposium.
\nContact persons:
for the scientific programme
: Å\;ke Forsberg
for the organisation: Eva-Maria Diehl