National and International Seminar Series 2014
\nSpeak
er:
Lars Bode
UCSD\, USA
Title: tba<
br />
Room: Betula\, bldg 6M
National and International Seminar Series 2 014
\nSpeaker:
Lars Bode
UCSD\, USA
Title: tba
Room: Betula\, bldg 6M
SciLifeLab will launch two Outreach Days \;per year with
the aim to raise awareness of the technologies and expertise that are&nbs
p\;offered at the SciLifeLab platforms to \;researchers from all of Sw
eden.? \;?
SciLifeLab scientists will visit universities
around Sweden\, two at each occasion\, starting with Umeå and Göteborg. Th
e first Outreach Day will focus on \;Genomics\, Bioinformatics and Dru
g Discovery and \;Development with other platforms presenting their ac
tivities in the form of posters.
The same day also the dire
ctors and platform coordinators of the National Genomics Infrastru
cture (NGI) will inform about NGI and local PIs will present exam
ples of their research and collaboration with NGI.
Date: 14 October 2014\, 9.30-16.30
Place: KBC\, Stora hörsalen\, KB3
B1 and KB3B3
Please register to the event here:
Registration form
P
rogramme \;
\;
\nContact: Eva-Maria Di
ehl\, This email address
is being protected from spambots. You need JavaScript enabled to view it.<
/span><
/p>
DTSTART;TZID=Europe/Stockholm:20141014T000000
DTEND;TZID=Europe/Stockholm:20141014T235959
SUMMARY:SciLifeLab Outreach Day 14 October\, KBC Umeå University
URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/163-events/3
66-scilifelab-outreach-day-14-october-kbc-umea-university.html
X-ALT-DESC;FMTTYPE=TEXT/HTML: SciLifeLab will launch two Outreach Days&nb
sp\;per year with the aim to raise awareness of the technologies and exper
tise that are \;offered at the SciLifeLab platforms to \;researche
rs from all of Sweden.? \;?
SciLifeLab scientists will vi
sit universities around Sweden\, two at each occasion\, starting with Umeå
and Göteborg. The first Outreach Day will focus on \;Genomics\, Bioin
formatics and Drug Discovery and \;Development with other platforms pr
esenting their activities in the form of posters.
The same
day also the directors and platform coordinators of the National G
enomics Infrastructure (NGI) will inform about NGI and local PIs
will present examples of their research and collaboration with NGI.
Date: 14 October 2014\, 9.30-16.30
Place: KBC\, Sto
ra hörsalen\, KB3B1 and KB3B3
Please register to the event he
re:
Registration form
Programme \;
\;
\nCont act: Eva-Maria Diehl\, Th is email address is being protected from spambots. You need JavaScript ena bled to view it.
END:VEVENT BEGIN:VEVENT UID:20141021T131500UTC-5bd117@http://www.ucmr.umu.se/ DTSTAMP:20260416T050958Z CATEGORIES:Seminar DESCRIPTION:
Medical Biochemistry and BiophysicsSeminar
Speaker:
Lee Makowski
Northeas
tern University\, Boston (MA)\, USA.
Title:
X-ray sol
ution scattering characterization of the structural ensemble of adenylate
kinase
Host: Magnus Wolf-Watz
\nRoom Lilla hörsalen \, KB3A9
\n—————————
DTSTART;TZID=Europe/Stockholm:20141021T151500 DTEND;TZID=Europe/Stockholm:20141021T161500 SUMMARY:Seminar - Lee Makowski: X-ray solution scattering characterization of the structural ensemble of adenylate kinase URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/164-seminar/ 372-seminar-lee-makowski-x-ray-solution-scattering-characterization-of-the -structural-ensemble-of-adenylate-kinase.html X-ALT-DESC;FMTTYPE=TEXT/HTML:
Medical Biochemistry and Biop
hysics
Seminar
Speaker:
Lee Makowski
Northeastern University\, Boston (MA)\, USA.
Title:
X-ray solution scattering characterization of the structural ensem
ble of adenylate kinase
Host: Magnus Wolf-Watz
\nRo om Lilla hörsalen\, KB3A9
\n—————————
END:VEVENT BEGIN:VEVENT UID:20141023T130000UTC-1a6236@http://www.ucmr.umu.se/ DTSTAMP:20260416T050958Z CATEGORIES:Seminar DESCRIPTION:KBC / UCMR Seminar
Adam Olsson
McGill University\, Canada
Title:
'Acous
tic Sensing of Bacterium-Substratum Interfaces'
Room
: KB3B3\, KBC
Host: Madeleine Ramstedt
Abstract:
Acoustic Sensing of Bacterium-Substratum Interfaces
Adam L.J.
Olsson
McGill University
\;
Bacterial adhesion to
surfaces and subsequent biofilm formation is an important phenomenon in ma
ny areas including\, amongst others\, biomedical engineering\, food proces
sing and water treatment. \;Since biofilms essentially originate from
only a few initial bacterial colonizers\, understanding the mechanisms go
verning the initial bacterial adhesion event may help designing surfaces w
ith the ability to manipulate biofilm formation.
This present
ation explores the possibilities to utilize a quartz crystal microbalance
with dissipation monitoring (QCM-D) to acoustically sense the mechanical p
roperties of the bacterium-surface interface. The QCM-D is generally consi
dered a mass balance\, where a negative shift in the resonance frequency o
f a quartz crystal sensor is proportional to attached mass. However\, in t
he case of bacterial adhesion\, the surface attached bacterium possesses a
resonance frequency that couples to the oscillation of the sensor surface
. The resulting frequency shift of this “coupled resonance” is either nega
tive or positive\, depending on the ratio between QCM-D resonance frequenc
y and the bacterium resonance frequency which\, in turn\, is determined by
its mass and surface contact stiffness. Thus\, analyzing bacterial adhesi
on in QCM-D within the context of “coupled resonance” offers a unique oppo
rtunity to monitor mechanical properties of bacterium-surface contacts.
Since the quartz sensor is mounted in a temperature controlled
flow module\, and because change in resonance frequency of the sensor is m
onitored in real time\, it possible to follow dynamic changes of the bacte
rium-surface contact during both the initial adhesion event as well as dur
ing subsequent biofilm growth. Another important aspect of the method is t
hat the stiffness\, which is related to bond strength\, is investigated wi
thout detaching the bacteria from the surface\; hence the method is non-de
structive.
\;
\;
KBC / UCMR Seminar
Ada
m Olsson
McGill University\, Canada
Title:
'Acoustic Sensing of Bacterium-Substratum Interfaces'<
br />
Room: KB3B3\, KBC
Host: Madeleine Ramstedt
Abstract:
Acoustic Sensing of Bacterium-Substratum Interface
s
Adam L.J. Olsson
McGill University
\;
Bacte
rial adhesion to surfaces and subsequent biofilm formation is an important
phenomenon in many areas including\, amongst others\, biomedical engineer
ing\, food processing and water treatment. \;Since biofilms essential
ly originate from only a few initial bacterial colonizers\, understanding
the mechanisms governing the initial bacterial adhesion event may help des
igning surfaces with the ability to manipulate biofilm formation.
This presentation explores the possibilities to utilize a quartz crys
tal microbalance with dissipation monitoring (QCM-D) to acoustically sense
the mechanical properties of the bacterium-surface interface. The QCM-D i
s generally considered a mass balance\, where a negative shift in the reso
nance frequency of a quartz crystal sensor is proportional to attached mas
s. However\, in the case of bacterial adhesion\, the surface attached bact
erium possesses a resonance frequency that couples to the oscillation of t
he sensor surface. The resulting frequency shift of this “coupled resonanc
e” is either negative or positive\, depending on the ratio between QCM-D r
esonance frequency and the bacterium resonance frequency which\, in turn\,
is determined by its mass and surface contact stiffness. Thus\, analyzing
bacterial adhesion in QCM-D within the context of “coupled resonance” off
ers a unique opportunity to monitor mechanical properties of bacterium-sur
face contacts.
Since the quartz sensor is mounted in a temper
ature controlled flow module\, and because change in resonance frequency o
f the sensor is monitored in real time\, it possible to follow dynamic cha
nges of the bacterium-surface contact during both the initial adhesion eve
nt as well as during subsequent biofilm growth. Another important aspect o
f the method is that the stiffness\, which is related to bond strength\, i
s investigated without detaching the bacteria from the surface\; hence the
method is non-destructive.
\;
\;
National and International Seminar Series
Speaker:
Pierre-Yves Lozach
Dept o
f Infectious Diseases\, Virology\, Univ.Hospital Heidelberg\, Germany
Title:
Bunyaviruses: host-to-host and cell-to-cell
Host: Anna Överby\, ClinMi
\n\;
\nRoom: \ ; Betula Lecture Hall\, Bldg 6M\, NUS
DTSTART;TZID=Europe/Stockholm:20141024T150000 DTEND;TZID=Europe/Stockholm:20141024T160000 SUMMARY:Nat. and Internat Seminar Series - Pierre-Yves Lozach: Bunyaviruses : host-to-host and cell-to-cell URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/164-seminar/ 370-nat-and-internat-seminar-series-pierre-yves-lozach-bunyaviruses-host-t o-host-and-cell-to-cell.html X-ALT-DESC;FMTTYPE=TEXT/HTML:
National and International Se
minar Series
Speaker:
Pierre-Yves Lozach
Dept of Infectious Diseases\, Virology\, Univ.Hospital Heidelbe
rg\, Germany
Title:
Bunyaviruses: host-to-host and ce
ll-to-cell
Host: Anna Överby\, ClinMi
\n\;
\nRoom: \; Betula Lecture Hall\, Bldg 6M\, NUS
END:VEVENT BEGIN:VEVENT UID:20141024T130000UTC-1f424f@http://www.ucmr.umu.se/ DTSTAMP:20260416T050958Z CATEGORIES:General DESCRIPTION:Department of Molecular Biology
\nThesis Defence
Constance Oben Ayuk Enow
Title:
Stud
ies of pore-forming bacterial protein toxins in Escherichia coli<
/strong>
Faculty Examiner: Mikael Rehn\, professor\, institu
tet för mikrobiologi\, tumör- och cellbiologi\, Karolinska Institutet
Supervisor: Bernt Eric Uhlin
\n\;
\nLecture room E 0 4\, Unod R1
DTSTART;TZID=Europe/Stockholm:20141024T150000 DTEND;TZID=Europe/Stockholm:20141024T160000 SUMMARY:Thesis Defence - Constance Oben Ayuk Enow URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/165-general/ 371-thesis-defence-constance-oben-ayuk-enow.html X-ALT-DESC;FMTTYPE=TEXT/HTML:Department of Molecular Biology
\nThe
sis Defence
Constance Oben Ayuk Enow
Title:< br />Studies of pore-forming bacterial protein toxins in Esche richia coli
\n
Faculty Examiner: Mikael Rehn\, pr
ofessor\, institutet för mikrobiologi\, tumör- och cellbiologi\, Karolinsk
a Institutet
Supervisor: Bernt Eric Uhlin
\n\;
\nLecture room E 04\, Unod R1
END:VEVENT BEGIN:VEVENT UID:20141028T141500UTC-c417cb@http://www.ucmr.umu.se/ DTSTAMP:20260416T050958Z CATEGORIES:Seminar DESCRIPTION:Department of Medical Biochemistry and Biophysics
\nSe minar Series
\nSpeaker:
Dr. Esther Bullitt
Dept. of Physiology &\; Biophysics\, Boston University School of Medi
cine\, Boston\, MA\, USA.
Title:
\n'Assembly Interme diates of the Shigella Type III Secretion Apparatus: Nascent and Primed Sy ringe Tips'
\nHosts: Bernt Eric Uhlin &\; Magnus Anderss on
\nRoom KB3A9\, \; Lilla hörsalen\, KBC
DTSTART;TZID=Europe/Stockholm:20141028T151500 DTEND;TZID=Europe/Stockholm:20141028T161500 SUMMARY:Esther Bullitt: Assembly Intermediates of the Shigella Type III Sec retion Apparatus: Nascent and Primed Syringe Tips URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/164-seminar/ 373-esther-bullitt-assembly-intermediates-of-the-shigella-type-iii-secreti on-apparatus-nascent-and-primed-syringe-tips.html X-ALT-DESC;FMTTYPE=TEXT/HTML:Department of Medical Biochemistry and Biop hysics
\nSeminar Series
\nSpeaker:
Dr. Esther Bul
litt
Dept. of Physiology &\; Biophysics\, Boston Universi
ty School of Medicine\, Boston\, MA\, USA.
Title:
\n'Assembly Intermediates of the Shigella Type III Secretion Apparatus: Nasc ent and Primed Syringe Tips'
\nHosts: Bernt Eric Uhlin & \; Magnus Andersson
\nRoom KB3A9\, \; Lilla hörsalen\, KBC
END:VEVENT BEGIN:VEVENT UID:20141030T140000UTC-fe43fa@http://www.ucmr.umu.se/ DTSTAMP:20260416T050958Z CATEGORIES:Seminar DESCRIPTION:Next generation tools for studying UPEC pathogenesis
\nSpeaker: MD/PhD Swaine Chen\, Singapore NRF Fellow\, Nat ional University of Singapore and Genome Institute of Singapore\, Singapor e.
\nTitle: 'Next generation tools for studying UPEC pathoge nesis'
\nRoom: Major Groove
\nHosts: Fredrik Almqvist and Sven Bergström \;
\nAbstract: Urinary tract infections (UTI s) are extremely common infections affecting half of all women and contrib uting greatly to antibiotic prescriptions and therefore bacterial antibiot ic resistance. Most UTIs are caused by uropathogenic Escherichia coli (UPE C). The study of how UPEC cause UTIs is greatly facilitated by many geneti c and molecular tools available for E. coli in general. However\, these to ols are usually developed in and for cloning or lab-adapted strains of E. coli\, and they are sometimes not usable or not efficient in disease-causi ng clinical isolates such as UPEC.
\nMy lab has developed two new to ols with clinical strains in mind to improve our ability to study UTI. The first improves our ability to manipulate the UPEC chromosome. We have dev eloped a general and modular negative selection (counterselection) system that functions without optimization in multiple clinical isolates of E. co li and Salmonella. Furthermore\, this system functions up to 1000x better than all other reported systems in lab strains of E. coli. This system now enables the convenient creation of definitive genetic constructs directly in UPEC.
\nThe second project improves our ability to detect UPEC d uring infection. We have designed a new GFP protein which we term vGFP tha t demonstrates a 30-50% improvement in bulk brightness while simultaneousl y enabling rational control of dimerization state. vGFP is therefore a bet ter chromosomal reporter than other GFP variants and should improve our de tection sensitivity of UPEC\, which is paramount for tracking UPEC as they move through minor niches during infection.
\nTogether these tools improve our ability to translate our mouse studies into human disease. I w ill conclude with our plans for performing niche-specific\, single-cell re solved\, simultaneous host and pathogen transcriptional profiling in direc t UTI samples. In the future\, this will hopefully allow us to do a full g enomic translation of our lab studies into knowledge about human disease.< /p> DTSTART;TZID=Europe/Stockholm:20141030T150000 DTEND;TZID=Europe/Stockholm:20141030T160000 SUMMARY:Seminar - Swaine Chen: Next generation tools for studying UPEC path ogenesis URL:http://www.ucmr.umu.se/about-ucmr/events/162-ucmr-calendar/164-seminar/ 368-seminar-swaine-chen-next-generation-tools-for-studying-upec-pathogenes is.html X-ALT-DESC;FMTTYPE=TEXT/HTML:
Next generation tools for studying UPEC pathogenesis
\nSpeaker: MD/PhD Swaine Chen\, Singapore NRF Fellow\, National University of Singapore and Genome Institute of Sin gapore\, Singapore.
\nTitle: 'Next generation tools for stud ying UPEC pathogenesis'
\nRoom: Major Groove
\nHosts: Fredrik Almqvist and Sven Bergström \;
\nAbstract: Urinary trac t infections (UTIs) are extremely common infections affecting half of all women and contributing greatly to antibiotic prescriptions and therefore b acterial antibiotic resistance. Most UTIs are caused by uropathogenic Esch erichia coli (UPEC). The study of how UPEC cause UTIs is greatly facilitat ed by many genetic and molecular tools available for E. coli in general. H owever\, these tools are usually developed in and for cloning or lab-adapt ed strains of E. coli\, and they are sometimes not usable or not efficient in disease-causing clinical isolates such as UPEC.
\nMy lab has dev eloped two new tools with clinical strains in mind to improve our ability to study UTI. The first improves our ability to manipulate the UPEC chromo some. We have developed a general and modular negative selection (counters election) system that functions without optimization in multiple clinical isolates of E. coli and Salmonella. Furthermore\, this system functions up to 1000x better than all other reported systems in lab strains of E. coli . This system now enables the convenient creation of definitive genetic co nstructs directly in UPEC.
\nThe second project improves our ability to detect UPEC during infection. We have designed a new GFP protein which we term vGFP that demonstrates a 30-50% improvement in bulk brightness wh ile simultaneously enabling rational control of dimerization state. vGFP i s therefore a better chromosomal reporter than other GFP variants and shou ld improve our detection sensitivity of UPEC\, which is paramount for trac king UPEC as they move through minor niches during infection.
\nToge ther these tools improve our ability to translate our mouse studies into h uman disease. I will conclude with our plans for performing niche-specific \, single-cell resolved\, simultaneous host and pathogen transcriptional p rofiling in direct UTI samples. In the future\, this will hopefully allow us to do a full genomic translation of our lab studies into knowledge abou t human disease.
END:VEVENT END:VCALENDAR